Part:BBa_K1462080:Design

TOM22

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Tom22 is anchored to the outer membrane by its hydrophobic segment in the middle of the sequence and it can carry the precursor protein into the channel formed by Tom40, the β-barrier protein which precursor protein can cross. Now we utilize the presequence of embed protein Tom22 and try to positioned our scaffold protein on the outer mitochondrial membrane.

Source

Tom22 is the basic presequence of S.cerevisae. Its sequence is from NCBI.